Part:BBa_K137113:Experience

Experience of UW-Madison 2010

The following is a zip file containing pertinent testing protocols and results, sequencing, and genbank files: media:BBa_K318500_pSB1C3.zip

Figure 1: L-Fucose content of plasmids K318500, K318501(K200029), and K200021 (control) in backbone pSB1AK3 transformed in MG1655. Colonic Acid is a polysaccharide containing a repeat unit with D-glucose, L-fucose, D-galactose, and D-glucuronate. Colonic acid can be estimated by measuring L-fucose content.

Figure 2: Survivability of plasmids K318500, K318501(K200029), K318502, and K200021 (control) in backbone pSB1AK3 transformed in MG1655. We found different results compared to the survivability testing preformed by Imperial 2009. We saw around a 10x increase in survivability with RcsB being over produced at a pH=4.

For more detail on testing, please see our results page on our Team Wiki for protocols, background information, and conclusions.

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